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pLX304-ERBB2

Plasmid

#175846

Purpose

Expresses ERBB2

Depositor

Evan Braunstein

Article

Braunstein et al Leukemia. 2016 Nov;30(11):2242-2245. doi

Insert

ERBB2 (ERBB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA3

Plasmid

#26679

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 3 (KPNA3 Human)

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-M

Plasmid

#38131

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-M (MITF Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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p201B Cas9

Plasmid

#59177

Purpose

Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
BAR

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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FLHKI-pGFPN3

Plasmid

#21917

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Full length human HKI in pGFP-N3 (HK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is full length human HKI in pGFP-N3

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH c-Met

Plasmid

#86498

Purpose

Mammalian expression of human c-Met

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Expression

Mammalian

Promoter

EF1a

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8r

Plasmid

#208318

Purpose

Overxpression MBP-TadA8r

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Ring1bCD

Plasmid

#235574

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ring1b (Rnf2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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CD9-mEGFP

Plasmid

#182864

Purpose

Localization: Tetraspanin/Membrane

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

human CD9 (CD9 Human)

Expression

Mammalian

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGH8 - pRAB-3::mCherry::unc-54utr

Plasmid

#19359

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Prab-3::mCherry

Tags

mCherry

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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RLuc_IRES_FLuc_Pless

Plasmid

#226462

Purpose

Promoterless bicistronic luciferase

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

Insert

RLuc IRES FLuc

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK-IDH1-R132H-FLAG

Plasmid

#66803

Purpose

Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

R132H

Promoter

CMV

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pSico p53

Plasmid

#12089

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating p53 shRNA expression.

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

p53 shRNA (Trp53 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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GST-RBD

Plasmid

#15247

Depositor

Martin Schwartz

Article

Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

Insert

Rhotekin (Rtkn Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 7-89 (Rho binding domain)

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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EcNR2 Strain

Bacterial Strain

#26931

Depositor

George Church

Article

Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

Bacterial Resistance

Chloramphenicol and Ampicillin

Available Since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
LentiV_Neo_SIK3_CR

Plasmid

#108104

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

change guanine 501 to cytosine (silent mutation)

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (AAV9)

Viral Prep

#100835-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (#100835). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only
pAAV-Neo_CAG-Cas9

Plasmid

#86698

Purpose

Cas9 expression vector for targeting to the AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

pCag

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]

Plasmid

#130909

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV8

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato (codon diversified version)

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

CAG

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pC3.1_CAG_oROS-Gr

Plasmid

#216113

Purpose

Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.01.31.578117

Insert

CAG_oROS-Gr

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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