164,713 results

1. p5E-mpeg1.1

   Plasmid

   #75023

   Purpose

   Can be used to drive expression in zebrafish microglia.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
   

   Use

   5' entry vector for multisite gateway three …

   Tags

   Expression

   Mutation

   Promoter

   mpeg1.1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-FGFR1c-V5/HIS

   Plasmid

   #201106

   Purpose

   expression of human FGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TagBFP2-FKBP-PIP5K1C_Kina-HD

   Plasmid

   #202741

   Purpose

   expresses recruitable fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mTagBFP2:FKBP1A(3-108):[GGSA]4GG:PIP5K1C(79-366)(D101R/R304D) (PIP5K1C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TagBFP2-FKBP-PIP5K1C_Kina-HDD316K

   Plasmid

   #202742

   Purpose

   expresses recruitable fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mTagBFP2:FKBP1A (3-108) : [GGSA]4GG : PIP5K1C(79-366) (D101R / R304D / D316K) (PIP5K1C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-FLEX-RiboL1-jGCaMP8s

   Plasmid

   #192616

   Purpose

   AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   FLEX-RIboL1-jGCaMP8s
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-Sec16A

   Plasmid

   #36155

   Purpose

   Expresses Sec16A as a GFP fusion

   Depositor

   David Stephens

   Article

   Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

   Insert

   Sec16A (SEC16A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. hCas9_D10A

   Plasmid

   #41816

   Purpose

   Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9_D10A
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A nickase

   Promoter

   CMV

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Noggin

   Plasmid

   #163586

   Purpose

   Expresses Noggin in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Nog (Nog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX301

   Plasmid

   #25895

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV/D374Y-hPCSK9

    Plasmid

    #58379

    Purpose

    Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

    Depositor

    Jacob Bentzon

    Article

    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

    Insert

    proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    D374Y

    Promoter

    HCRApoE/hAAT

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. mTagBFP2-Rab5a-7

    Plasmid

    #55322

    Purpose

    Localization: Ras GTPase, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Rab5a
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pSLIK_neo_3xflag_CHEK2

    Plasmid

    #136536

    Purpose

    Lentiviral expression vector for an inducible 3xflag-tagged CHEK2

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    CHEK2 (CHEK2 Human)
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-beta-catenin-S33Y

    Plasmid

    #13371

    Purpose

    Retroviral expression of mouse beta-catenin S33Y mutant

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    catenin (cadherin associated protein), beta 1 (S33Y mutant) (Ctnnb1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Serine 33 to Tyrosine and Leucine 388 to Proline

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.3-NL63-D14

    Plasmid

    #172666

    Purpose

    encodes NL63 spike protein, for pseudovirus production

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    HCNV63gp2 (HCNV63gp2 human coronavirus NL63)
    

    Use

    Lentiviral and Retroviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    c-terminal deletion of 14aa

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2-hspDRv7_scGstlt

    Plasmid

    #108870

    Purpose

    scGESTALT V7 lineage barcode for zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Insert

    scGESTALT lineage tracing target V7
    

    Use

    Tol2 integration into zebrafish genome

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCMV Puro DEST P38KTRDronpa

    Plasmid

    #205763

    Purpose

    Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    P38 KTR Dronpa
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. mApple-M1 Spastin

    Plasmid

    #134461

    Purpose

    M1 Spastin expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met to Ala change at amino acid 87

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMP71-IFN-gamma

    Plasmid

    #174602

    Purpose

    Constitutive expression of secreted murine IFN gamma

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    Constitutive expression of secreted murine IFN gamma
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid

    #36047

    Purpose

    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA(R118G)-HA
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    Available since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-GCaMP6fu

    Plasmid

    #86235

    Purpose

    Express fluorescent calcium indicator GCaMP6fu in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2016 Dec 6;6:38276. doi: 10.103

    Insert

    GCaMP6fu
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W43Y D395A

    Promoter

    CMV

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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