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180,111 results

Showing: 8561 - 8580 of 180111 results

  1. PARP1 FL

    Plasmid
    #169815
    Purpose
    Expresses codon optimised full length His tagged PARP1 in bacterial cells
    Depositor
    David Neuhaus
    Publication
    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226
    Insert
    Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Human, Synthetic)
    Tags
    MKHHHHHHMKQ
    Expression
    Bacterial
    Mutation
    Valine 762 mutated to an alanine
    Promoter
    T7 promoter
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    169815-plasmid-map-sequence-id-334563

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  3. Gi1-CASE

    Plasmid
    #168120
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A121/E122 within GNAI1
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    168120-plasmid-map-sequence-id-332858

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  4. pAAV-Ef1a-Coff/Fon-GCaMP6F (AAV8)

    Viral prep
    #137124-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-GCaMP6F (#137124). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-GCaMP6F plasmid DNA. EF1a-driven, Flp-dependent expression of GCaMP6f (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Available since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  8. pMS2M-PH-gagpol-D64V

    Plasmid
    #166031
    Purpose
    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.
    Depositor
    Yujia Cai
    Publication
    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038
    Insert
    MS2-Gag-Pol
    Use
    Lentiviral
    Mutation
    D64V mutation within the integrase within Pol
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    166031-plasmid-map-sequence-id-328290

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  9. LifeAct-mGreenLantern

    Plasmid
    #164459
    Purpose
    Localization of mGreenLantern fluorescent protein to actin
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mGreenLantern
    Tags
    LifeAct
    Expression
    Mammalian
    Mutation
    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…
    Promoter
    CMV
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    164459-plasmid-map-sequence-id-322382

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  10. pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE

    Plasmid
    #162382
    Purpose
    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Has service
    AAV1, AAV5, AAV9, and AAV Retrograde
    Insert
    jGCaMP8f
    Use
    AAV and Cre/Lox
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Q315L
    Promoter
    CAG
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    162382-plasmid-map-sequence-id-318382

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  11. pcDNA3-mem-TurboID

    Plasmid
    #149409
    Purpose
    Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection
    Depositor
    Jonathan Long
    Publication
    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mem-TurboID
    Tags
    HA and V5
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    149409-plasmid-map-sequence-id-299724

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  12. AAVS1-tdTomato

    Plasmid
    #159275
    Purpose
    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11
    Insert
    tdTomato
    Use
    AAV; S1 knockin donor vector
    Expression
    Mammalian
    Promoter
    CAGGS
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    159275-plasmid-map-sequence-id-312908

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  13. 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid
    #158652
    Purpose
    making transgenics expressing a UAS driven eGFP and NTR 2.0
    Depositor
    Jeff Mumm
    Publication
    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    Use
    Tol2-based transgenic expression in zebrafish/oth…
    Mutation
    F70A/F108Y
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    158652-plasmid-map-sequence-id-310824

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  16. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

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Showing: 8561 - 8580 of 180111 results