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(Plasmid #26245)


Item Catalog # Description Quantity Price (USD)
Plasmid 26245 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Lutz and Bujard, Nucleic Acids Research 25 (1997) 1203-1210.
  • Backbone size w/o insert (bp) 3659
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
    MS2 coat protein and GFP fusion
  • Species
  • Insert Size (bp)
  • Mutation
    five amino acid linker (Ser, Gly, Gly, Gly, Gly) between the C terminus of the dlFG mutant MS2 coat protein and the N terminus of GFP. The MS2 coat protein is a dlFG mutant with a deletion for amino acids 68-82.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer PLlacO-1
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    MS2-GFP fusion constructed in Le et al PNAS 2005 (also from Cluzel lab). pZS*12 vector was a gift from H. Bujard to C. Guet.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZS*12-MS2-GFP was a gift from Philippe Cluzel (Addgene plasmid # 26245 ; ; RRID:Addgene_26245)
  • For your References section:

    Minimally invasive determination of mRNA concentration in single living bacteria. Guet CC, Bruneaux L, Min TL, Siegal-Gaskins D, Figueroa I, Emonet T, Cluzel P. Nucleic Acids Res. 2008 Jul . 36(12):e73. 10.1093/nar/gkn329 PubMed 18515347