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pZE31-DsRed-ms2x2
(Plasmid #26248)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26248 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pZE31-DsRed
  • Backbone manufacturer
    constructed in Cluzel Lab
  • Backbone size w/o insert (bp) 2600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ms2 binding sites (x2)
  • Insert Size (bp)
    200

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (destroyed during cloning)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer PLtetO-1
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The tandem ms2 binding sites were cloned from the pIIIMS2-2 plasmid, which was a gift from K Bloom and UNC Chapel Hill. DsRedT3f was cloned from pQE31-DsRed.T3f from Dan Strongnin and the Glick Lab, U Chicago The vector was a gift from H. Bujard to C. Guet.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZE31-DsRed-ms2x2 was a gift from Philippe Cluzel (Addgene plasmid # 26248)
  • For your References section:

    Minimally invasive determination of mRNA concentration in single living bacteria. Guet CC, Bruneaux L, Min TL, Siegal-Gaskins D, Figueroa I, Emonet T, Cluzel P. Nucleic Acids Res. 2008 Jul . 36(12):e73. 10.1093/nar/gkn329 PubMed 18515347