|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25860||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerLutz and Bujard, Nucleic Acids Research 25 (1997) 1203-1210.
- Backbone size w/o insert (bp) 2000
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
Copy numberHigh Copy
Gene/Insert namems2 binding site (x2)
Insert Size (bp)150
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer PLtetO-1 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byms2 binding sequence was amplified from pIIIMS2-2 plasmid from K Bloom UNC Chapel Hill. Vector provided to C. Guet from H. Bujard.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pZE31ms2 was a gift from Philippe Cluzel (Addgene plasmid # 25860 ; http://n2t.net/addgene:25860 ; RRID:Addgene_25860)
For your References section:Real-time RNA profiling within a single bacterium. Le TT, Harlepp S, Guet CC, Dittmar K, Emonet T, Pan T, Cluzel P. Proc Natl Acad Sci U S A. 2005 Jun 28. 102(26):9160-4. 10.1073/pnas.0503311102 PubMed 15967986