pRK5myc RhoA L63
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15900||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameA isoform of Rho
SpeciesH. sapiens (human)
Insert Size (bp)600
Entrez GeneRHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
/ Fusion Protein
- myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Constitutively activated Rho.
There is an additional F25N mutation in the sequence. This change greatly facilitated expression of the protein in E.coli. The only known effect of this mutation may be negatively affect the transduction of RhoA signaling to DGK1 kinase. Otherwise the depositing lab have never found any discrepancies with other published work on activity or protein interactions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK5myc RhoA L63 was a gift from Alan Hall (Addgene plasmid # 15900 ; http://n2t.net/addgene:15900 ; RRID:Addgene_15900)
For your References section:Rho GTPases control polarity, protrusion, and adhesion during cell movement. Nobes CD, Hall A. J Cell Biol. 1999 Mar 22. 144(6):1235-44. 10.1083/jcb.144.6.1235 PubMed 10087266