|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26292||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerVerma lab
- Backbone size w/o insert (bp) 8753
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Insert Size (bp)627
Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer dsRed1-
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
From the paper: To construct pTomo H-RasV12, we amplified Flag-tagged H-RasV12 from pGEM H-RasV12 by PCR and inserted it into the BamHI site of the pTomo vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTomo-Ras was a gift from Inder Verma (Addgene plasmid # 26292)
For your References section:Development of a novel mouse glioma model using lentiviral vectors. Marumoto T, Tashiro A, Friedmann-Morvinski D, Scadeng M, Soda Y, Gage FH, Verma IM. Nat Med. 2009 Jan . 15(1):110-6. 10.1038/nm.1863 PubMed 19122659