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pTomo-Ras
(Plasmid #26292)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26292 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTomo
  • Backbone manufacturer
    Verma lab
  • Backbone size w/o insert (bp) 8753
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HRAS-V12
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    627
  • Mutation
    G12V
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer dsRed1-
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

From the paper: To construct pTomo H-RasV12, we amplified Flag-tagged H-RasV12 from pGEM H-RasV12 by PCR and inserted it into the BamHI site of the pTomo vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTomo-Ras was a gift from Inder Verma (Addgene plasmid # 26292)
  • For your References section:

    Development of a novel mouse glioma model using lentiviral vectors. Marumoto T, Tashiro A, Friedmann-Morvinski D, Scadeng M, Soda Y, Gage FH, Verma IM. Nat Med. 2009 Jan . 15(1):110-6. 10.1038/nm.1863 PubMed 19122659