|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26291||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonep156RRLsin PPTCMVIRESPRE
Backbone manufacturerNaldini lab
- Backbone size (bp) 8753
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
To generate pTomo, the inserted loxP-mRFP-loxP fragment amplified from the pSETB mRFP1 vector by PCR between the XbaI and BamHI sites of the p156RRLsin PPTCMVIRESPRE vector (a third-generation lentiviral vector).
Note that the Addgene quality control sequence detected a G->A mutation in the IRES. It is not known if or how this mutation affects function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTomo was a gift from Inder Verma (Addgene plasmid # 26291 ; http://n2t.net/addgene:26291 ; RRID:Addgene_26291)
For your References section:Development of a novel mouse glioma model using lentiviral vectors. Marumoto T, Tashiro A, Friedmann-Morvinski D, Scadeng M, Soda Y, Gage FH, Verma IM. Nat Med. 2009 Jan . 15(1):110-6. 10.1038/nm.1863 PubMed 19122659