|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16624||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 5099
Vector typeMammalian Expression
Growth in Bacteria
SpeciesH. sapiens (human)
Entrez GeneBUB1 (a.k.a. BUB1A, BUB1L, hBUB1)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer EBV rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
pBI-GFP-Bub1 WT was constructed by cloning RT-PCR products representing the WT hBUB1 sequence into the NotI and SalI sites of pBI-GFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBI-GFP-Bub1-wt was a gift from Bert Vogelstein (Addgene plasmid # 16624 ; http://n2t.net/addgene:16624 ; RRID:Addgene_16624)
For your References section:Mutations of mitotic checkpoint genes in human cancers. Cahill DP, Lengauer C, Yu J, Riggins GJ, Willson JK, Markowitz SD, Kinzler KW, Vogelstein B. Nature. 1998 Mar 19. 392(6673):300-3. 10.1038/32688 PubMed 9521327