Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pBI-GFP-Bub1-wt
(Plasmid #16624)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16624 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBI-GFP
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 5099
  • Vector type
    Mammalian Expression
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BUB1
  • Alt name
    Bub1
  • Species
    H. sapiens (human)
  • Entrez Gene
    BUB1 (a.k.a. BUB1A, BUB1L, hBUB1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EBV rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

pBI-GFP-Bub1 WT was constructed by cloning RT-PCR products representing the WT hBUB1 sequence into the NotI and SalI sites of pBI-GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBI-GFP-Bub1-wt was a gift from Bert Vogelstein (Addgene plasmid # 16624 ; http://n2t.net/addgene:16624 ; RRID:Addgene_16624)
  • For your References section:

    Mutations of mitotic checkpoint genes in human cancers. Cahill DP, Lengauer C, Yu J, Riggins GJ, Willson JK, Markowitz SD, Kinzler KW, Vogelstein B. Nature. 1998 Mar 19. 392(6673):300-3. 10.1038/32688 PubMed 9521327