|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26588||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Speciesmammalian codon optimized
Insert Size (bp)345
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
iLOV codon usage was optimized for mammalian cell expression by de novo gene synthesis (GenScript) and cloned into pEGFP-N1 (Clontech) via SalI and NotI to replace the GFP encoding sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEiLOV-N1 was a gift from John Christie (Addgene plasmid # 26588 ; http://n2t.net/addgene:26588 ; RRID:Addgene_26588)
For your References section:The photoreversible fluorescent protein iLOV outperforms GFP as a reporter of plant virus infection. Chapman S, Faulkner C, Kaiserli E, Garcia-Mata C, Savenkov EI, Roberts AG, Oparka KJ, Christie JM. Proc Natl Acad Sci U S A. 2008 Dec 16. 105(50):20038-43. 10.1073/pnas.0807551105 PubMed 19060199