Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26587||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGE Healthcare
- Backbone size w/o insert (bp) 4900
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameImproved Lov
Alt nameformerly Phot2
SpeciesA. thaliana (mustard weed)
Insert Size (bp)330
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pGEX-5
- 3′ sequencing primer pGEX-3 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX iLOV was a gift from John Christie (Addgene plasmid # 26587 ; http://n2t.net/addgene:26587 ; RRID:Addgene_26587)
For your References section:The photoreversible fluorescent protein iLOV outperforms GFP as a reporter of plant virus infection. Chapman S, Faulkner C, Kaiserli E, Garcia-Mata C, Savenkov EI, Roberts AG, Oparka KJ, Christie JM. Proc Natl Acad Sci U S A. 2008 Dec 16. 105(50):20038-43. 10.1073/pnas.0807551105 PubMed 19060199