164,599 results

1. pJ404-DogCatcher-sfGFP

   Plasmid

   #171930

   Purpose

   Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   DogCatcher-sfGFP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mini-human AsCpf1-based human genome-wide knockout library

   Pooled Library

   #130630

   Purpose

   Expresses crRNAs for genome-wide knockout pooled library screening

   Depositor

   Giulio Draetta

   Article

   Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

3. pTRIPZ shSTING-Hsa

   Plasmid

   #128158

   Purpose

   Doxycyclin inducible shRNA knockdown of human STING gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shSTING Hsa (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2,0_3M5M_eGFP

   Plasmid

   #135474

   Purpose

   Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   Marburg virus minigenome, eGFP reporter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   T7

   Available since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

   Plasmid

   #183409

   Purpose

   piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Piggybac-TetO-hNurr1-blast

   Plasmid

   #176485

   Purpose

   TetO-hNurr1 in a PiggyBac vector with Blasticidin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   Nurr1 (NR4A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

   Plasmid

   #198240

   Purpose

   For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

   Depositor

   Didier Stainier

   Article

   Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

   Inserts

   Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
   Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
   

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-RdLight1 (AAV9)

   Viral Prep

   #125707-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-CAG-RdLight1 (#125707). In addition to the viral particles, you will also receive purified pAAV-CAG-RdLight1 plasmid DNA. CAG-driven expression of red genetically encoded dopamine sensor RdLight1. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

   Promoter

   CAG

   Tags

   None

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

9. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

   Plasmid

   #64216

   Purpose

   Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA targeting ROSA26-1
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. GST-RH▲-MNase

    Plasmid

    #136292

    Purpose

    Expression of catalytic inactive RNase H fused to micrococcal nuclease

    Depositor

    Kavitha Sarma

    Article

    Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

    Insert

    RNase HI-D10R-E48R and MNase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-RhoA Biosensor

    Plasmid

    #68026

    Purpose

    C-terminus of Anillin in mammalian expression vector pEGFP-C1

    Depositor

    Michael Glotzer

    Article

    Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

    Insert

    Anillin (AHD+PH) (ANLN Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. bdSUMO-GFP-HiBit

    Plasmid

    #170989

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRES-EGFP-puro

    Plasmid

    #45567

    Depositor

    Michael McVoy

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    IRES-EGFP-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Phcmv (strong human cytomegalovirus immediate ear…

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH_Str-Ii

    Plasmid

    #65313

    Purpose

    Cytoplasmic ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Invariant chain (p33)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Streptavidin in its cytoplasmic part

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTH3

    Plasmid

    #84453

    Purpose

    shuttle plasmid for sarA P1-YFP expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    YFP venus
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV PHP.eB)

    Viral Prep

    #100854-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Promoter

    Syn

    Tags

    None

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

17. Human Lipid Droplet and Metabolism Library

    Pooled Library

    #191535

    Purpose

    Lentiviral CRISPR library targeting 1,196 genes related to lipid droplet biology and cell metabolism.

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

18. AAV phSyn1(S)-tdTomato-WPRE (AAV5)

    Viral Prep

    #51506-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from AAV phSyn1(S)-tdTomato-WPRE (#51506). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-tdTomato-WPRE plasmid DNA. hSyn-driven tdTomato expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Promoter

    phSyn1

    Tags

    tdTomato

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pMRX-IP TFEB-sfGFP

    Plasmid

    #135402

    Purpose

    Expresses TFEB-sfGFP

    Depositor

    Eisuke Itakura

    Article

    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

    Insert

    TFEB (TFEB Human)
    

    Use

    Retroviral

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221-ZNF208

    Plasmid

    #101549

    Purpose

    Donor Vector containing ZNF208 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF208 (ZNF208 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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