Synthetic single domain antibodies for the conformational trapping of membrane proteins.
Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmur S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA
Elife. 2018 May 24;7. pii: 34317. doi: 10.7554/eLife.34317. PubMed Article
Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmur S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA
Elife. 2018 May 24;7. pii: 34317. doi: 10.7554/eLife.34317. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110098 | pBXNPH3 | E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage site |
| 110099 | pBXNPHM3 | E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage site |
| 110100 | pSBinit | E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTag |
| 110101 | pDXinit | E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages |
| 110102 | SB_concave | pBXPHM3 with scaffold Sybody of the concave library |
| 110103 | SB_loop | pBXPHM3 with scaffold Sybody of the loop library |
| 110104 | SB_convex | pBXPHM3 with scaffold Sybody of the convex library |