CRISPR artificial splicing factors.
Du M, Jillette N, Zhu JJ, Li S, Cheng AW
Nat Commun. 2020 Jun 12;11(1):2973. doi: 10.1038/s41467-020-16806-4. PubMed Article
Du M, Jillette N, Zhu JJ, Li S, Cheng AW
Nat Commun. 2020 Jun 12;11(1):2973. doi: 10.1038/s41467-020-16806-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118634 | pAC1801_pmax-dCasRx | Transient transfection; Expresses dCasRx; CAGGS promoter |
| 118635 | pAC1802_pmax-RBFOX1N-dCasRx-C | Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter |
| 118637 | pAC1803_pmax-RBM38-dCasRx | Transient transfection; Expresses RBM38-dCasRx; CAGGS promoter |
| 118638 | pAC1804_pmax-dCasRx-RBM38 | Transient transfection; Expresses dCasRx-RBM38; CAGGS promoter |
| 118639 | pAC1806_pmax-FKBP-dCasRx | Transient transfection; Expresses FKBP-dCasRx; CAGGS promoter |
| 118640 | pAC1807_pmax-dCasRx-FKBP | Transient transfection; Expresses dCasRx-FKBP; CAGGS promoter |
| 118641 | pAC1808_pmax-RBFOX1N-FRB-C | Transient transfection; Expresses RBFOX1N-FRB-C; CAGGS promoter |
| 118642 | pAC1809_pmax-RBM38-FRB | Transient transfection; Expresses RBM38-FRB; CAGGS promoter |
| 118643 | pAC1810_pmax-FRB-RBM38 | Transient transfection; Expresses FRB-RBM38; CAGGS promoter |
| 118645 | pAC1812_pCR8-gCasRx-CUG (control gRNA) | Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor |
| 118646 | pAC1813_pCR8-gSMN2-DN1 | Transient transfection; Expressed SMN2-DN1 CasRx gRNA; Gateway Donor |
| 118647 | pAC1814_pCR8-gSMN2-DN2 | Transient transfection; Expressed SMN2-DN2 CasRx gRNA; Gateway Donor |
| 118648 | pAC1815_pCR8-gSMN2-DN3 | Transient transfection; Expressed SMN2-DN3 CasRx gRNA; Gateway Donor |
| 118649 | pAC1816_pCR8-gSMN2-DN4 | Transient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway Donor |
| 118651 | pAC1817_pCR8-gSMN2-EX | Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor |
| 118652 | pAC1818_pCR8-gRG6-SA | Transient transfection; Expressed RG6-SA CasRx gRNA; Gateway Donor |
| 118653 | pAC1819_pCR8-DR-SMN2-2-DR | Transient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor |
| 118654 | pAC1820_pCR8-DR-RG6-SA-DR | Transient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor |
| 118655 | pAC1821_pCR8-SMN2-DN-RG6-SA | Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor |
| 118656 | pAC1822_pCR8-dCasRx | Gateway Donor; dCasRx ORF; No promoter |
| 118657 | pAC1823_pCR8-RBFOX1N-dCasRx-C | Gateway Donor; RBFOX1N-dCasRx-C ORF; No promoter |
| 118662 | pAC1825_pCR8-dCasRx-RBM38 | Gateway Donor; dCasRx-RBM38 ORF; No promoter |