|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||118635||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
- Promoter CAGGS
- Cloning method Gateway Cloning
- 5′ sequencing primer gggcttgtcgagacagagaagat
- 3′ sequencing primer ACCGAGGAGAGGGTTAGGGAT (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bydCasRx coding sequence from Konermann et al (doi:10.1016/j.cell.2018.02.033) XR002: EF1a-dCasRx-2A-EGFP (Plasmid #109050); Effector sequence cloned using IDT gBlock as template
Terms and Licenses
More info at http://CasFx.org . Please visit https://www.biorxiv.org/content/early/2018/09/30/431064 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC1802_pmax-RBFOX1N-dCasRx-C was a gift from Albert Cheng (Addgene plasmid # 118635 ; http://n2t.net/addgene:118635 ; RRID:Addgene_118635)
For your References section:CRISPR Artificial Splicing Factors. Jillette N, Cheng AW. bioRxiv 431064 /10.1101/431064