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A high-avidity biosensor reveals plasma membrane PI(3,4)P2 is predominantly a class I PI3K signaling product.
Goulden BD, Pacheco J, Dull A, Zewe JP, Deiters A, Hammond GRV
J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi: 10.1083/jcb.201809026. Epub 2018 Dec 27.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
116853NES-EGFP-cPHx1PI(3,4)P2 biosensor
116854NES-EGFP-cPHx2PI(3,4)P2 biosensor
116855NES-EGFP-cPHx3PI(3,4)P2 biosensor
116856TagBFP2-CAAXplasma membrane marker
116857TagBFP2-INPP4B-CAAXplasma membrane-targetted PI(3,4)P2 4-phosphatase
116858TagBFP2-INPP4BC842A-CAAXinactive plasma membrane targetted PI(3,4)P2 phosphatase
116859NES-PAmCherry-cPHx1PI(3,4)P2 biosensor
116860NES-PAmCherry-cPHx2PI(3,4)P2 biosensor
116861NES-PAmCherry-cPHx3PI(3,4)P2 biosensor
116862NES-mCherry-cPHx3PI(3,4)P2 biosensor
116863pNES-iRFP-C1cytosolic iRFP
116864mCherry-FKBP-INPP4BRecruitable PI(3,4)P2 4-phosphatase
116865mCherry-FKBP-INPP4BC842AInactive recruitable PI(3,4)P2 4-phosphatase
116866mCherry-FKBP-PTENRecruitable PTEN dual-specificity phosphatase
116868NES-EGFP-PH-ARNO2G-I303Ex2PIP3 biosensor

Antibodies from Article