A high-avidity biosensor reveals plasma membrane PI(3,4)P2 is predominantly a class I PI3K signaling product.
Goulden BD, Pacheco J, Dull A, Zewe JP, Deiters A, Hammond GRV
J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi: 10.1083/jcb.201809026. Epub 2018 Dec 27. PubMed Article
Goulden BD, Pacheco J, Dull A, Zewe JP, Deiters A, Hammond GRV
J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi: 10.1083/jcb.201809026. Epub 2018 Dec 27. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 116853 | NES-EGFP-cPHx1 | PI(3,4)P2 biosensor |
| 116854 | NES-EGFP-cPHx2 | PI(3,4)P2 biosensor |
| 116855 | NES-EGFP-cPHx3 | PI(3,4)P2 biosensor |
| 116856 | TagBFP2-CAAX | plasma membrane marker |
| 116857 | TagBFP2-INPP4B-CAAX | plasma membrane-targetted PI(3,4)P2 4-phosphatase |
| 116858 | TagBFP2-INPP4BC842A-CAAX | inactive plasma membrane targetted PI(3,4)P2 phosphatase |
| 116859 | NES-PAmCherry-cPHx1 | PI(3,4)P2 biosensor |
| 116860 | NES-PAmCherry-cPHx2 | PI(3,4)P2 biosensor |
| 116861 | NES-PAmCherry-cPHx3 | PI(3,4)P2 biosensor |
| 116862 | NES-mCherry-cPHx3 | PI(3,4)P2 biosensor |
| 116863 | pNES-iRFP-C1 | cytosolic iRFP |
| 116864 | mCherry-FKBP-INPP4B | Recruitable PI(3,4)P2 4-phosphatase |
| 116865 | mCherry-FKBP-INPP4BC842A | Inactive recruitable PI(3,4)P2 4-phosphatase |
| 116866 | mCherry-FKBP-PTEN | Recruitable PTEN dual-specificity phosphatase |
| 116868 | NES-EGFP-PH-ARNO2G-I303Ex2 | PIP3 biosensor |