TagBFP2-INPP4BC842A-CAAX
(Plasmid #116858)

• Purpose: inactive plasma membrane targetted PI(3,4)P2 phosphatase
• Depositing Lab: Gerry Hammond
• Publication: Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079. doi: 10.1083/jcb.201809026. Epub 2018 Dec 27.

Backbone

• Vector backbone: pTagBFP2-C1
• Total vector size (bp): 7530
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: INPP4B
• Species: H. sapiens (human)
• Insert Size (bp): 2772
• Mutation: catalytic cysteine 842 changed to alanine
• Entrez Gene: INPP4B
• Tags / Fusion Proteins:
  • TagBFP2 (N terminal on insert)
  • HRAS(172-189) (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-For
• 3′ sequencing primer: SV40-pArev

Resource Information

• Supplemental Documents:
  • pTagBFP2-C1-INPP4B-C842A-CAAX.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/09/06/410811 for bioRxiv preprint.