327,302 results

1. pCAMBgfp

   Plasmid

   #206130

   Purpose

   This binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.

   Depositor

   Anne Osbourn

   Article

   Sesma et al Nature. 2004 Sep 30;431(7008):582-6. doi

   Insert

   hygromycin resistance gene and the Promoter of ToxA+SGFP
   

   Use

   Binary vector for expression of sgfp in filamento…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTol1-UAS:ChrimsonR-tdTomato

   Plasmid

   #124231

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   ChrimsonR-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   14xUAS-E1b

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol1-UAS:ChrimsonR-tdTomato

   Plasmid

   #124231

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   ChrimsonR-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   14xUAS-E1b

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-cell-mCer

   Plasmid

   #185601

   Purpose

   Lentiviral construct for expression of mCerulean

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Insert

   cell-mCer
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-cell-mCer

   Plasmid

   #185601

   Purpose

   Lentiviral construct for expression of mCerulean

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Insert

   cell-mCer
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIMAY

   Plasmid

   #68939

   Purpose

   E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

   Depositor

   Tim Foster

   Article

   Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

   Type

   Empty backbone

   Use

   Staphylococcal allelic exchange vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIMAY

   Plasmid

   #68939

   Purpose

   E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

   Depositor

   Tim Foster

   Article

   Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

   Type

   Empty backbone

   Use

   Staphylococcal allelic exchange vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. EphA2-YFP

   Plasmid

   #108852

   Purpose

   Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

   Depositor

   Kalina Hristova

   Article

   Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

   Insert

   EPHA2 (EPHA2 Human)
   

   Use

   Tags

   Labeled with eYFP on the C-Terminus via a 15 amin…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. EphA2-YFP

   Plasmid

   #108852

   Purpose

   Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

   Depositor

   Kalina Hristova

   Article

   Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

   Insert

   EPHA2 (EPHA2 Human)
   

   Use

   Tags

   Labeled with eYFP on the C-Terminus via a 15 amin…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRIS-PITChv2-dTAG-BSD (BRD4)

    Plasmid

    #91795

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRIS-PITChv2-dTAG-BSD (BRD4)

    Plasmid

    #91795

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57.1_V5_TurboID_FLAG_Ct

    Plasmid

    #194073

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-TurboID-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57.1_V5_TurboID_FLAG_Ct

    Plasmid

    #194073

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-TurboID-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (AAV1)

    Viral Prep

    #100833-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (#100833). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    Syn

    Tags

    None

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

17. T7-CMVtrans-FFLuc-polyA

    Plasmid

    #101156

    Purpose

    Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

    Depositor

    Marcel Bruchez

    Article

    Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. T7-CMVtrans-FFLuc-polyA

    Plasmid

    #101156

    Purpose

    Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

    Depositor

    Marcel Bruchez

    Article

    Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbA2c-RFP

    Plasmid

    #35326

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbA2c-RFP

    Plasmid

    #35326

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge