164,674 results

1. p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

   Plasmid

   #216479

   Purpose

   lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   wtYAP (YAP1) (YAP1 Human)
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1aL and UBC

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330S-2

   Plasmid

   #58778

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-PIK3CB

   Plasmid

   #23542

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PIK3CB (PIK3CB Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   I6L

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pLenti-EF1a-Backbone(NG)

   Plasmid

   #27963

   Depositor

   Feng Zhang

   Article

   Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

   Insert

   TAL-Backbone(NG)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-GfaABC1D-GRAB_ATP1.0mut

   Plasmid

   #167580

   Purpose

   Express the ATP-insensitive control sensor GRAB_ATP1.0mut in astrocytes

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GfaABC1D

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-ZM197M-gp160

   Plasmid

   #123226

   Purpose

   Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (ZM197M) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKIIa-hChR2(H134R)-EYFP

   Plasmid

   #26969

   Purpose

   AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

   Has Service

   AAV1, AAV2, AAV5, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Histidine 134 is mutated to Arginine

   Promoter

   CaMKIIa

   Available since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. HIP-flash (HOP-flash mutant)

   Plasmid

   #83466

   Purpose

   Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

   Depositor

   Barry Gumbiner

   Article

   Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

   Insert

   Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7II-2N4Rtau

    Plasmid

    #177653

    Purpose

    Recombinant protein synthesis of human Tau

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    Tau (2N4R) (MAPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYLCRISPR/Cas9Pubi-H

    Plasmid

    #66187

    Purpose

    expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    maize ubiquitin promoter (Pubi)

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT2/shp53/GFP4

    Plasmid

    #20208

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    shp53/GFP4
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pG1

    Plasmid

    #162602

    Purpose

    Edit Ade2 gene in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9 with RPL25 Intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWT35S-Citrine-NLS

    Plasmid

    #182583

    Purpose

    Transient expression of Citrine-NLS in plant cell (Nucleus)

    Depositor

    Yutaka Kodama

    Article

    Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

    Insert

    NLS
    

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. ABCA1 promoter luciferase reporter (P1 fragment)

    Plasmid

    #86443

    Purpose

    Human ABCA1 promoter luciferase reporter (P1 fragment)

    Depositor

    David Mu

    Article

    Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

    Insert

    Human ABCA1 (ABCA1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD649-HAsp-VSTM2L-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156582

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    VSTM2L (VSTM2L Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-ERBB2-v2

    Plasmid

    #154895

    Purpose

    Expresses ERBB2 (a.k.a HER2) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Tags

    Venus fragment 2 (v2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57.1 DOX off blast NFS1

    Plasmid

    #160801

    Purpose

    Express Dox repressible NFS1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    NFS1 (NFS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPHK2 gRNA (BRDN0001146242)

    Plasmid

    #77095

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN7-S15

    Plasmid

    #190665

    Purpose

    Expresses N7 ribosomal protein S15

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7 S15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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