164,713 results

1. AAV-hSyn-RiboL1-jGCaMP8m

   Plasmid

   #169248

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   RiboL1-jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-GFP

   Plasmid

   #16664

   Depositor

   Fred Gage

   Article

   Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

   Insert

   eGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

   Plasmid

   #185686

   Purpose

   Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-GST-HaloTag-KKMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-myc-OGA(GS-544)C181-D174N

   Plasmid

   #193999

   Purpose

   plasmid encoding myc-tagged OGA(GS-544)C181-D174N

   Depositor

   Christina Woo

   Article

   Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

   Insert

   myc-OGA(GS-544)C181-D174N
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF1096

   Plasmid

   #143760

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   EN2 (EN2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-RalGDS

   Plasmid

   #118315

   Purpose

   Expression of human RalGDS

   Depositor

   Philip Stork

   Article

   Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

   Insert

   RalGDS (RALGDS Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   fragment of human RalGDS 203aa

   Promoter

   CMV

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBbi-Neo

   Plasmid

   #60525

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. PT2/C-Luc//PGK-SB13

   Plasmid

   #20207

   Depositor

   John Ohlfest

   Article

   Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

   Insert

   Flirefly luciferase/Sb transposase
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. cr3Dd

   Plasmid

   #184862

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, bet, exo
   

   Use

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-Q842gp160

    Plasmid

    #100931

    Purpose

    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Use

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmiR-report-MYB-mut

    Plasmid

    #141115

    Purpose

    Reporter plasmid of mutant MYB in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYB (MYB Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    A to T mutations at m6A sites

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. EcM2.1

    Bacterial Strain

    #64052

    Purpose

    Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

    Depositor

    George Church

    Article

    Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

    Bacterial Resistance

    Ampicillin

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pMXs-mGCN2-FLAG-IP

    Plasmid

    #101794

    Purpose

    Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

    Depositor

    Seiichi Oyadomari

    Article

    Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

    Insert

    Gcn2 (Eif2ak4 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQC V5 MCS IRES G418

    Plasmid

    #110345

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Geneticin selection.

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. 7xSMAD_RE::FLuc

    Plasmid

    #124531

    Purpose

    SMAD2 FLuc luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    SMAD FLuc reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3/UBC9

    Plasmid

    #20082

    Depositor

    Edward Yeh

    Article

    Gong et al J Biol Chem. 1997 Nov 7;272(45):28198-20

    Insert

    UBC9 (UBE2I Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-SpyCas9

    Plasmid

    #113033

    Purpose

    SpyCas9 expression in mammalian cells (CMV promoter driven)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    SpyCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSH424-ssr

    Plasmid

    #198026

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Sm resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SmR promoter

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG

    Plasmid

    #225245

    Purpose

    Mammalian expression of iPASnFR-H in the nucleus

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-JNKKTRClover

    Plasmid

    #59139

    Purpose

    ORF encoding JNK KTR mClover flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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