Fourth-generation epac-based FRET sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated sensors for FLIM, for ratiometry and with high affinity.
Klarenbeek J, Goedhart J, van Batenburg A, Groenewald D, Jalink K
PLoS One. 2015 Apr 14;10(4):e0122513. doi: 10.1371/journal.pone.0122513. eCollection 2015. PubMed Article
Klarenbeek J, Goedhart J, van Batenburg A, Groenewald D, Jalink K
PLoS One. 2015 Apr 14;10(4):e0122513. doi: 10.1371/journal.pone.0122513. eCollection 2015. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170341 | Epac-S-H94 | GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 170345 | Epac-S-H150 | 6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 170347 | Epac-S-H159 | mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 170348 | Epac-S-H187 | mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 170349 | Epac-S-H188 | mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 170350 | Epac-S-H189 | mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 170352 | Epac-S-H170 | mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |
| 170353 | Epac-S-H171 | mTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells. |