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An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo.
Vallese F, Catoni C, Cieri D, Barazzuol L, Ramirez O, Calore V, Bonora M, Giamogante F, Pinton P, Brini M, Cali T
Nat Commun. 2020 Nov 27;11(1):6069. doi: 10.1038/s41467-020-19892-6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
164107SPLICS Mt-ER Long P2ADetect the long Mitochondria-Endoplasmic reticulum contact
164108SPLICS Mt-ER Short P2ADetect the short Mitochondria-Endoplasmic reticulum contact
164109SPLICS Mt-ER- PM Long P2ADetect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact
164110SPLICS Mt-ER- PM Short P2ADetect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact
164111SPLICS PM-ER Long P2ADetect the long Plasma membrane-Endoplasmic reticulum contact
164112SPLICS PM-ER Short P2ADetect the short Plasma membrane-Endoplasmic reticulum contact
164113SPLICS Po-ER Long P2ADetect the long Peroxisomes-Endoplasmic reticulum contact
164114SPLICS Po-ER Short P2ADetect the long Peroxisomes-Endoplasmic reticulum contact
164115SPLICS Po-Mt Long P2ADetect the long Peroxisomes-mitochondria contact
164116SPLICS Po-Mt Short P2ADetect the short Peroxisomes-mitochondria contact
164117SPLICS Mt-ER- Po Long P2ADetect the long Peroxisomes-mitochondria-Endoplasmic reticulum contact
164118SPLICS Mt-ER- Po Short P2ADetect the short Peroxisomes-mitochondria-Endoplasmic reticulum contact

Antibodies from Article