180,111 results
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Plasmid#73187PurposeExpresses "control"-APEX (cytosolic) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcontrol-APEX (Nphp3 Mouse, Glycine max)
Tagsinternal GFP tagExpressionMammalianMutationchanged glycine 2 to alaninePromoterEF1alphaAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
C321.deltaA (Isaacs lab)
Bacterial strain#73581PurposeGenomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB geneDepositorBacterial resistanceAmpicillinAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-flex-dsRed-shvgat
Plasmid#67845PurposeExpresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switchDepositorInsertAAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA
UseAAV, Cre/Lox, Mouse Targeting, and RNAiExpressionMammalianPromoterh-synapsinAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-FLAG-Sestrin2
Plasmid#72595PurposeoverexpressionDepositorInsertSESN2 (SESN2 Human)
TagsFLAGExpressionMammalianMutationcodon optimized for mammalian expression, see dep…PromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MMK
Plasmid#64121Purposea modified episomal (EBNA1/OriP) vector expressing human Myc and KLF4 genesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterSFFVAvailable sinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ133A
Plasmid#69275PurposeGolden Gate entry vector; 3rd gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLsgRNA-OsU6b
Plasmid#66196Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterOsU6bAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-KDEL_SBP-mCherry-Ecadherin
Plasmid#65287Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEcadherin
TagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS-FuG-E
Plasmid#67509PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.DepositorInsertFusion Glycoprotein type E
UseLentiviralTagsFusion protein of Vesicular stomatitis Indiana vi…PromoterCAGGSAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST26-USP47
Plasmid#64326PurposeMammalian expression and purification of USP47.DepositorAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 iTEV
Plasmid#64277PurposeExpresses infrared fluorescent tobacco etch virus protease sensor (iTEV)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInfrared fluorescent tobacco etch virus protease sensor (iTEV)
ExpressionMammalianPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAB1
Plasmid#62547Purposebroad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.DepositorInserteGFP
UseBroad host range plasmidExpressionBacterialPromoterpTrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-BP
Plasmid#60496PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHL-EF1a-SphcCas9-iP-A
Plasmid#60599PurposeExpresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRISPR Cas9
UseCRISPRExpressionMammalianMutationCodon usage optimized for human usageAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-B19L
Plasmid#59922PurposeExpression vector for Rabies virus SAD B19 RNA dependent RNA polymerase geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRabies virus RNA dependent RNA polymerase
ExpressionMammalianPromoterCAGAvailable sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV phSyn1(S)-tdTomato-WPRE
Plasmid#51506PurposeCan be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoterDepositorHas serviceAAV5InserttdTomato
UseAAVPromoterphSyn1Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mKate1.31-pBAD
Plasmid#54827PurposeLocalization: Protein Expression Vector , Excitation: 588, Emission: 635. Also known as mKate2.DepositorTypeEmpty backboneTags6xHis and mKate1.31 (aka mKate2)ExpressionBacterialAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC119-MCS
Plasmid#58807Purposecontains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.DepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEmerald-Desmin-C-18
Plasmid#54059PurposeLocalization: Intermediate Filaments, Excitation: 487, Emission: 509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDesmin (DES Human)
TagsmEmeraldExpressionMammalianPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by