167,825 results

1. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

   Plasmid

   #170378

   Purpose

   Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

   Depositor

   Jeremy Day

   Article

   Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

   Insert

   A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   human Synapsin promoter

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Opie2-PuroR

   Plasmid

   #131613

   Purpose

   Expresses PuroR and dsRed.

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

   Inserts

   PuroR
   dsRed
   

   Expression

   Insect

   Promoter

   Opie2 and hr5Ie1

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MCP-M-MLV RT∆RNase H

   Plasmid

   #213749

   Purpose

   For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TSC2-fl

   Plasmid

   #24940

   Depositor

   Kunliang Guan

   Insert

   TSC2 (Tsc2 Mouse)
   

   Use

   N/a

   Available Since

   Sept. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

5. mCherry-BP1-2 pLPC-Puro

   Plasmid

   #19835

   Depositor

   Titia de Lange

   Article

   Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

   Insert

   Fragment of p53-Binding Protein 1 (TP53BP1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Mutation

   Fragment of human 53BP1 aa 1220 - 1711

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. FTO-dCas9

   Plasmid

   #134781

   Purpose

   Expression fusion protein FTO-dCas9 in Mammalian cells

   Depositor

   Shu-Bing Qian

   Article

   Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

   Insert

   FTO and inactive dCas9
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer10-mir-RUP-PheS

   Plasmid

   #44011

   Purpose

   Lentiviral shRNA vector (pGIPz based) for inducible knockdown

   Depositor

   Stephen Elledge

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Insert

   PheS Gly294
   

   Use

   Lentiviral

   Tags

   RFP

   Expression

   Mammalian

   Promoter

   TRE2

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBJ6

   Plasmid

   #167135

   Purpose

   A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   Kanamycin promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Rabbit GFP Cav 2.2 pMT2

   Plasmid

   #58737

   Purpose

   expression of GFP tagged rabbit Cav2.2 in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

   Insert

   Cav2.2 (CACNA1B Rabbit)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCL1271

    Plasmid

    #217963

    Purpose

    Mammalian expression of Importin 5 (Imp5, IPO5) with an N-terminal eGFP fusion. Splice isoform 1.

    Depositor

    Andrew Bowman

    Article

    Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

    Insert

    Imp5 (IPO5 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pULTRA-CNF

    Plasmid

    #48215

    Purpose

    highly efficient suppressor plasmid

    Depositor

    Peter Schultz

    Article

    Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

    Inserts

    polyspecific MJ tyrosyl synthetase
    tRNA
    

    Expression

    Bacterial

    Mutation

    Y32L, L65V, F108W, Q109M, D158G, I159A

    Promoter

    proK and tacI

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRE-ChrimsonR-mCherry

    Plasmid

    #92207

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    ChimsonR-mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_STAT3_p.A662C_N664C

    Plasmid

    #82184

    Purpose

    Gateway Donor vector containing STAT3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Gateway entry vector

    Mutation

    A662C, N664C

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_FGF1_WT

    Plasmid

    #81825

    Purpose

    Gateway Donor vector containing FGF1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    FGF1 (FGF1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPHR-mCherry-hNPM

    Plasmid

    #181887

    Purpose

    Soluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-hNPM (NPM1 Human, Mustard Weed)
    

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGST-PSP-hTG

    Plasmid

    #85470

    Purpose

    bacterial expression of human tissue transglutaminase

    Depositor

    Ray Truant, Jeffrey Keillor

    Article

    Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

    Insert

    hTG2 (TGM2 Human)
    

    Tags

    GST and PSP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. TMPRSS2

    Plasmid

    #53887

    Purpose

    Expression of TMPRSS2

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Has Service

    Cloning Grade DNA

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pYLCRISPR/Cas9P35S-B

    Plasmid

    #66190

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. psPax2-D64V-NC-mNeon

    Plasmid

    #196509

    Purpose

    mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    mNeon
    

    Tags

    mNeon

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVSV-G

    Plasmid

    #138479

    Purpose

    Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    VSV-G envelop protein
    

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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