167,846 results

1. pcDNA-ncpGCaMP6s

   Plasmid

   #113674

   Purpose

   A topological variant of GCaMP6s

   Depositor

   Robert Campbell

   Article

   Qian et al Chembiochem. 2018 Jun 22. doi: 10.1002/c

   Insert

   ncpGCaMP6s
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. RBM10_pLX307

   Plasmid

   #98371

   Purpose

   Lentiviral expression of RBM10

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   RBM10 (RBM10 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Isoform 2

   Promoter

   E1Fa

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMG059 MBP-CREB-His

   Plasmid

   #154076

   Purpose

   Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   CREB1 (CREB1 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-hPPP3CB-FLAG

   Plasmid

   #179135

   Purpose

   Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLBS-mScarlet

   Plasmid

   #129337

   Purpose

   cytosol marker, lentivirus

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   mScarlet (Addgene 85042)
   

   Use

   Lentiviral

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

   Plasmid

   #213118

   Purpose

   Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   PKAsub-mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUP-(2xCouRS-tRNAopt)

   Plasmid

   #197930

   Purpose

   Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

   Inserts

   CouRS
   tRNA opt
   

   Expression

   Bacterial

   Promoter

   glnS' and lambda(ts) and proK

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1 beta-catenin WT

   Plasmid

   #16071

   Depositor

   Erin Schuman

   Article

   Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

   Insert

   beta-catenin (CTNNB1 Chicken)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   *

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE-8XGTIIC-DsRED-DD

   Plasmid

   #115798

   Purpose

   lentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter

   Depositor

   Joan Massague

   Article

   Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

   Insert

   YAP responsive promoter (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   DsRED-DHFR

   Expression

   Mammalian

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-multi-CRISPR

    Plasmid

    #85402

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-3xFLAG-TERT

    Plasmid

    #51631

    Purpose

    Mammalian expression of 3xFLAG tagged TERT, N-terminal.

    Depositor

    Steven Artandi

    Article

    Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

    Insert

    TERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1a-MinE-IRES-Blast

    Plasmid

    #213115

    Purpose

    Expression of untagged MinE in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE (minE )
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUB_smHA_KDM5B_MS2

    Plasmid

    #81085

    Purpose

    Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

    Depositor

    Tim Stasevich

    Article

    Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

    Insert

    smHA_KDM5B_24xMS2
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKRaION1-N1

    Plasmid

    #177801

    Purpose

    Mammalian expression of green fluorescent potassium indicator KRaION1

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDAI-SceI-pheS

    Plasmid

    #111611

    Purpose

    Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Insert

    pheS
    

    Expression

    Bacterial

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-GRP78-Wild-Type

    Plasmid

    #235169

    Purpose

    Expresses Wild-Type GRP78 in mammalian cells

    Depositor

    Amy Shiu Lee

    Article

    Zhang et al J Biol Chem. 2010 May 14;285(20):15065-1

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.2 TDP-43 NLS1 YFP

    Plasmid

    #84912

    Purpose

    Mammalian expresion of TDP-43 NLS1 YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

    Plasmid

    #179116

    Purpose

    Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    Cargo EGFP with AttP Bxb1 site for in-frame fusion
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

    Plasmid

    #79671

    Purpose

    Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

    Depositor

    Scott Sternson

    Article

    Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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