167,846 results

1. pAS1NB m Rosella I

   Plasmid

   #71247

   Purpose

   a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.

   Depositor

   Mark Prescott

   Article

   Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

   Insert

   Rosella
   

   Tags

   yeast mitochondrial targeting sequence of citrate…

   Expression

   Yeast

   Promoter

   PGK1

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-tmpknot-GG-acceptor

   Plasmid

   #174039

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Calnexin-HT8-mGold

   Plasmid

   #183989

   Purpose

   Expresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.

   Depositor

   Pablo Rivera-Fuentes

   Article

   Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

   Insert

   Calnexin (CANX Human)
   

   Tags

   HaloTag8 (C61S, C262S), mGold

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

   Plasmid

   #87259

   Purpose

   YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133

   Depositor

   David Sibley

   Article

   Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

   Inserts

   YFP-mAID-3HA
   HXGPRT
   

   Use

   Toxoplasma expression

   Tags

   3HA and mAID

   Mutation

   Codon optimized for Toxoplasma gondii expression

   Promoter

   TgDHFR-TS 463 bp and TgTUB1 378 bp

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. T-STOP sgRNA-1

   Plasmid

   #83346

   Purpose

   The sgRNA at the stop codon of human Brachyury(T) locus

   Depositor

   James Thomson

   Article

   Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

   Insert

   T-STOP sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-hCGN

   Plasmid

   #236507

   Purpose

   For expression of Cingulin (human) in mammalian cells

   Depositor

   Sandra Citi

   Article

   Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

   Insert

   CGN (CGN Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ601 - Peft-3 Mos1 transposase

   Plasmid

   #34874

   Purpose

   Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   Mos1 transpoase
   

   Use

   Worm targeting

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSWAP_Entry_HB

   Plasmid

   #184868

   Purpose

   pSwap plasmid part with HB homology arms

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pKD3_mCherry

   Plasmid

   #187385

   Purpose

   To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT

   Depositor

   Roi Avraham

   Insert

   mCherry
   

   Expression

   Bacterial

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Dendra2-CathespinB-6

    Plasmid

    #57703

    Purpose

    Localization: Lysosomes, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Insert

    CathespinB
    

    Tags

    Dendra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. repressilator (pZS1-lTlrLLtCL)

    Plasmid

    #26489

    Depositor

    Michael Elowitz

    Article

    Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

    Insert

    LacI, tetR
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pESC-TRP (V5 and HA)

    Plasmid

    #222488

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and TRP1 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Tags

    HA and V5

    Expression

    Yeast

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Halo-TOMM20-N-10

    Plasmid

    #123284

    Purpose

    Localization: Outer Mitochondrial Membrane

    Depositor

    Kevin McGowan

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid

    #216156

    Purpose

    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with C-terminal FLAG tag
    

    Expression

    Mammalian

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-MFSD6_STOP

    Plasmid

    #161119

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    MFSD6 (MFSD6 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pMSCV-Blasticidin

    Plasmid

    #75085

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-Cas9-NLS-6xHis

    Plasmid

    #62933

    Purpose

    Expression of Cas9-NLS-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9-NLS-6xHis
    

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. mRFP-Lact-C2

    Plasmid

    #74061

    Purpose

    Fluorescent biosensor for phosphatidylserine.

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

    Insert

    Lactadherin-C2 (MFGE8 Bovine)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. BS-His3

    Plasmid

    #69197

    Purpose

    complementation of yeast auxotrophic marker HIS3

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    HIS3
    

    Expression

    Yeast

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-EF1a-MinD-IRES-Puro

    Plasmid

    #213114

    Purpose

    Expression of untagged MinD in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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