167,846 results

1. pSG5-PBX1a

   Plasmid

   #78149

   Purpose

   mammalian expression of PBX1a

   Depositor

   Francesco Blasi

   Insert

   PBX1 (PBX1 Human)
   

   Expression

   Mammalian

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pFH3.3_Cre-MS2x12

   Plasmid

   #205559

   Purpose

   Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Cre
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBtet-Bla/GFP

   Plasmid

   #198221

   Purpose

   Transposon vector (Addgene #60510) for doxycycline-inducible expression of EGFP

   Depositor

   Eric Davis

   Insert

   EGFP
   

   Use

   Transposon

   Expression

   Mammalian

   Mutation

   None

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-HA-CDK4

   Plasmid

   #172612

   Purpose

   Expresses HA-tagged CDK4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK4 (CDK4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PT3.5/CMV-EGFRvIII

   Plasmid

   #20280

   Depositor

   John Ohlfest

   Article

   Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

   Insert

   EGFRvII (EGFR Human)
   

   Use

   Transposon

   Mutation

   See depositor comments below.

   Available Since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28b-RfxCas13d-His

   Plasmid

   #141322

   Purpose

   Plasmid for bacterial expression and purification of RfxCas13d protein

   Depositor

   Ariel Bazzini, Miguel Angel Moreno-Mateos

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-EGxxFP

   Plasmid

   #50716

   Purpose

   5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

   Depositor

   Masahito Ikawa

   Article

   Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

   Insert

   split EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 HA E2F1

   Plasmid

   #236431

   Purpose

   transient overexpression of E2F1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

   Insert

   E2F1 (E2F1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-HA-hTAF4b

   Plasmid

   #17998

   Depositor

   Robert Tjian

   Article

   Freiman et al Science. 2001 Sep 14. 293(5537):2084-7.

   Insert

   hTAF4b (TAF4B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa1-64 deleted

   Available Since

   May 28, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

    Plasmid

    #169992

    Purpose

    Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    TEV-siriusGFP-FLAG

    Expression

    Mammalian

    Mutation

    S297A

    Promoter

    CMV

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_SEPT9_i5-msfGFP

    Plasmid

    #180327

    Purpose

    mammalian expression of human SEPT9_i5 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v7 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-FLAG-S6K1

    Plasmid

    #100509

    Purpose

    Expresses FLAG tagged S6K1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PJEBAN4

    Plasmid

    #203683

    Purpose

    Expresses red fluorescent marker HcRed in Listeria monocytogenes

    Depositor

    Tine Rask Licht

    Article

    Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

    Insert

    HcRed
    

    Expression

    Bacterial

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. slc1a3b:myrGCaMP6s-P2A-H2AmCherry

    Plasmid

    #170209

    Purpose

    Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus

    Depositor

    Kelly Monk

    Article

    Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

    Insert

    slc1a3b (slc1a3b Zebrafish)
    

    Use

    Zebrafish

    Tags

    myrGCaMP6s-P2A-H2AmCherry

    Promoter

    slc1a3b

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-link-ymNeongreen-URA3

    Plasmid

    #168059

    Purpose

    Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeonGreen
    

    Tags

    ymNeonGreen

    Expression

    Yeast

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSFFV_mNG3A(1-10)

    Plasmid

    #157992

    Purpose

    Lentiviral vector of constitutive expression of mNeonGreen3A(1-10)

    Depositor

    Bo Huang

    Article

    Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

    Insert

    pSFFV_mNG3K(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    W148L, M150V, K153M, N194K, F204L

    Promoter

    SFFV

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. NW11

    Plasmid

    #133443

    Purpose

    Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW11
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-hSynI-rtTAV16

    Plasmid

    #102367

    Purpose

    AAV vector to drive TET-ON activator expression from human synapsin I promoter

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

    Insert

    rtTAV16
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    rtTA variant reported in Gene Therapy (2006) 13, …

    Promoter

    human synapsin I promoter

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPLICS Po-Mt Long P2A

    Plasmid

    #164115

    Purpose

    Detect the long Peroxisomes-mitochondria contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Long P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-CA

    Plasmid

    #20960

    Purpose

    piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Expression

    Mammalian

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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