164,728 results

1. GST-RH▲-MNase

   Plasmid

   #136292

   Purpose

   Expression of catalytic inactive RNase H fused to micrococcal nuclease

   Depositor

   Kavitha Sarma

   Article

   Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

   Insert

   RNase HI-D10R-E48R and MNase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

   Plasmid

   #225754

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

   Plasmid

   #157744

   Purpose

   knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   chlorotoxin containing chimeric antigen receptor targeting glioblastoma
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-Sac-Abeta(M1-40)

   Plasmid

   #71876

   Purpose

   Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-40) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFC334

   Plasmid

   #87846

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   argB
   yA specific sgRNA
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   A. nidulans gpdA promoter with Hammerhead ribozym…

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACUH

   Plasmid

   #58374

   Purpose

   UAS vector to express gene of interest in Drosophila

   Depositor

   Chun Han, Yuh-Nung Jan

   Article

   Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-HA-hIFITM1

   Plasmid

   #58399

   Purpose

   Expressed human IFITM1 with an N-terminal HA tag in mammalian cells

   Depositor

   Jacob Yount, Howard Hang

   Article

   Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

   Insert

   IFITM1 (IFITM1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLAMP1-miRFP670nano

   Plasmid

   #127435

   Purpose

   LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   LAMP1-miRFP670nano
   

   Use

   Tags

   miRFP670nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-6xHis-(-30)GFP

   Plasmid

   #62936

   Purpose

   Expression of 6xHis-(-30)GFP in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)GFP
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

    Plasmid

    #207860

    Purpose

    AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLminP_Luc2P_RE43

    Plasmid

    #90381

    Purpose

    RUNX - Runx1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    GATA3

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMN-333

    Plasmid

    #195467

    Purpose

    Lentiviral Integratable Inducible synZiFTR ZF3 + Reporter

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    inverted[8X ZF3 BS - ybTATA - mCherry] - pSFFV - ZF3 - p65 - ERT2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTagBFP2-Rab5a-7

    Plasmid

    #55322

    Purpose

    Localization: Ras GTPase, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Rab5a
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. mCherry-cGAS-HA

    Plasmid

    #231895

    Purpose

    Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    cGAS (CGAS Human)
    

    Use

    Tags

    3xHA and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. mRuby2-SiT-N-15

    Plasmid

    #55912

    Purpose

    Localization: Golgi TGN, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    SiT (ST6GAL1 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    aa 1-45 of SiT

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. gRNA_tdtomato_reporter_1_SA-2x

    Plasmid

    #79369

    Purpose

    Assays activity of transcriptional activators fused to SA Cas9

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    tdtomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJYS1Peftu

    Plasmid

    #85546

    Purpose

    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    etfu and unknown

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cdc2-HA

    Plasmid

    #1888

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdc2 (CDK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BII-CPspaH2B

    Plasmid

    #133385

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Il12b 400bp pro-Luc

    Plasmid

    #20020

    Depositor

    Stephen Smale

    Article

    Plevy et al Mol Cell Biol. 1997 Aug . 17(8):4572-88.

    Insert

    Il12b promoter (Il12b Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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