167,846 results

1. pLJM1-PM-RA-BlastR

   Plasmid

   #211708

   Purpose

   Lentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   PM-targetted RA (Lck Synthetic)
   

   Use

   Lentiviral

   Tags

   RA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Synapsin-cyto-iATPSnFR1.0

   Plasmid

   #102556

   Purpose

   expresses cytoplasmic ATP sensor in neurons

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.0
   

   Use

   AAV

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-EGFP-C1-HsYTHDF2_AB

   Plasmid

   #148310

   Purpose

   Mammalian Expression of HsYTHDF2

   Depositor

   Elisa Izaurralde

   Insert

   HsYTHDF2 (YTHDF2 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Su9-EGFP

   Plasmid

   #23214

   Depositor

   David Chan

   Article

   Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

   Insert

   Subunit 9 of the F0-ATPase
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains AA 1-69 of subunit 9

   Available Since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-CaNAR2

   Plasmid

   #64728

   Purpose

   FRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNAR2
   

   Tags

   Cerulean3 and YPet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF278

   Plasmid

   #167673

   Purpose

   Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cas12f-GE ver4.1

   Plasmid

   #176544

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver4.1
   Cas12f-GE ver4.1
   

   Expression

   Mammalian

   Promoter

   U6 and chicken β-actin

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV Retrograde)

   Viral Prep

   #55645-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent Channelrhodopsin-EYFP expression for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   Syn

   Tags

   EYFP (Cre- and Flp-dependent)

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pHMattR3-attR4-GFP

   Plasmid

   #196247

   Purpose

   pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUW-tetO-Pu.1

    Plasmid

    #139839

    Purpose

    doxycycline-inducible expression of mouse Pu.1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Pu.1 (Spi1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-P70a-Top-T~P70a-Bottom-T

    Plasmid

    #87314

    Purpose

    Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE30-His-T7RNAP

    Plasmid

    #124138

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    T7 RNA polymerase (T7p07 Enterobacteria phage T7)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. mOrange2-Fibrillarin-7

    Plasmid

    #57957

    Purpose

    Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Fibrillarin (FBL Human)
    

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pAAV_CMV::hTfR-GFP

    Plasmid

    #199714

    Purpose

    Expresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    hTfR-GFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cyto-RA

    Plasmid

    #209860

    Purpose

    Dimerization dependent fluorescent protein RA fused to NES

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Tags

    NES sequence NLVDLQKKLEELELDEQQ

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. miCBE

    Plasmid

    #205413

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with APOBEC3A and UGI driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pU6__RNA*_pCAG_bpNLS_APOBEC3A(W104A)_XTEN_OgeuIscB*(D61A)_NLS_2xUGI_bGHployA_pCMV_mCherry
    

    Expression

    Mammalian

    Mutation

    D61A+E85R+H369R+S387R+S457R

    Promoter

    hU6, CAG, CMV

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYHY2

    Plasmid

    #216108

    Purpose

    Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.

    Depositor

    Shannon Sirk

    Article

    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

    Insert

    NanoLuc
    

    Use

    Synthetic Biology; E. coli-bacteroides shuttle ve…

    Tags

    3xFLAG tag and BT0294 signal peptide

    Expression

    Bacterial

    Promoter

    BfP1E6 promoter

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRIPZ Myc2-mouse TTP WT

    Plasmid

    #107000

    Purpose

    Expresses epitope-tagged wild-type murine TTP

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    tristetraprolin (Zfp36 Mouse)
    

    Tags

    Double Myc peptide epitope tag

    Expression

    Mammalian

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p406GALL

    Plasmid

    #17434

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    GALL promoter is between SacI and XbaI restrictio…

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Casp8

    Plasmid

    #11817

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 8 (CASP8 Human)
    

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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