|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51005||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 9055
Modifications to backboneCut pLenti.CAG.GFP with SalI, blunt, then BamHI. Ligated pLenti.CAG (BamHI/SalI blunt) with H2B-Dendra2 insert (BglII, EcoRV)
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)1151
- Promoter CAG
/ Fusion Protein
- histone2B (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Sal1 (destroyed during cloning)
- 3′ cloning site BamH1 (destroyed during cloning)
- 5′ sequencing primer T3
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti.CAG.H2B-Dendra2.W was a gift from Rusty Lansford (Addgene plasmid # 51005 ; http://n2t.net/addgene:51005 ; RRID:Addgene_51005)