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(Plasmid #51005)


Item Catalog # Description Quantity Price (USD)
Plasmid 51005 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 9055
  • Modifications to backbone
    Cut pLenti.CAG.GFP with SalI, blunt, then BamHI. Ligated pLenti.CAG (BamHI/SalI blunt) with H2B-Dendra2 insert (BglII, EcoRV)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Promoter CAG
  • Tag / Fusion Protein
    • histone2B (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (destroyed during cloning)
  • 3′ cloning site BamH1 (destroyed during cloning)
  • 5′ sequencing primer T3
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti.CAG.H2B-Dendra2.W was a gift from Rusty Lansford (Addgene plasmid # 51005 ; ; RRID:Addgene_51005)