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p55-H2B-Dendra2
(Plasmid #80609)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80609 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p55
  • Backbone size w/o insert (bp) 4823
  • Total vector size (bp) 5936
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H2B-Dendra2
  • Alt name
    histone cluster 2, H2be
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1086
  • Entrez Gene
    H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
  • Promoter CAG
  • Tag / Fusion Protein
    • Dendra2 (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer cttctggcgtgtgaccgg
  • 3′ sequencing primer tagaaggcacagtcgagg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p55-H2B-Dendra2 was a gift from Periklis Pantazis (Addgene plasmid # 80609 ; http://n2t.net/addgene:80609 ; RRID:Addgene_80609)

  • For your References section:

    Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins. Mohr MA, Argast P, Pantazis P. Nat Protoc. 2016 Dec;11(12):2419-2431. doi: 10.1038/nprot.2016.134. Epub 2016 Nov 3. 10.1038/nprot.2016.134 PubMed 27809312
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