pLenti.CAG.H2B-cerFP-2A-mito-dsRFP.W
(Plasmid #51013)

• Purpose: Lentivector that expresses H2B-cerFP-2A-mito-dsRFP from internal CAG enhancer-promoter
• Depositing Lab: Rusty Lansford
• Publication: Lentivirus (unpublished)

Backbone

• Vector backbone: pLenti.CAG
• Backbone size w/o insert (bp): 7276
• Total vector size (bp): 9818
• Modifications to backbone: pLenti.CAG:eGFP digested w/ Sal1, blunt w Klenow, digested with BamH1 to remove GFP and gel isolated. Ligated with PCR generated H2B-cerFP-2A-mito-RFP after digesting with Bgl2-EcoRV and gel isolated.
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: H2B-cerFP-2A-mito-RFP
• Species: Synthetic
• Insert Size (bp): 2542
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (destroyed during cloning)
• 3′ cloning site: BamH1 (destroyed during cloning)
• 5′ sequencing primer: EGFP-N
• 3′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.