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Purposepromoter activity study
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Depositing Lab
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Depositing OrganizationUniversity of Alabama at Birmingham Research Foundation (Inactive/For Deposit Only)
Located in United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 53432-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 53432-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL2 basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5597
- Total vector size (bp) 7000
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMMP2 promoter
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Alt namematrix metallopeptidase 2
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SpeciesH. sapiens (human)
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Insert Size (bp)1400
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GenBank IDNM-001127891
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Entrez GeneMMP2 (a.k.a. CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1)
- Promoter MMP2
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Tag
/ Fusion Protein
- luciferase (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer LucNrev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 53432-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 53432-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
human MMP-2 promoter was a gift from Etty Benveniste (Addgene plasmid # 53432 ; http://n2t.net/addgene:53432 ; RRID:Addgene_53432) -
For your References section:
The transcription factors Sp1, Sp3, and AP-2 are required for constitutive matrix metalloproteinase-2 gene expression in astroglioma cells. Qin H, Sun Y, Benveniste EN. J Biol Chem. 1999 Oct 8;274(41):29130-7. PubMed 10506168
