|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58807||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerMessing Lab
- Backbone size (bp) 3345
Vector typePlant Expression, CRISPR
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
All restriction sites between EcoRI and HindIII are single-cut except AscI. Multiple gRNA expression cassettes cloned within the MCSs between two AscI sites can be subcloned into the AscI site of pFGC-pcoCas9 binary plasmid (Addgene plasmid#52256) through one-step cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC119-MCS was a gift from Jen Sheen (Addgene plasmid # 58807 ; http://n2t.net/addgene:58807 ; RRID:Addgene_58807)