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pUC119-MCS
(Plasmid #58807)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58807 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC119
  • Backbone manufacturer
    Messing Lab
  • Backbone size (bp) 3345
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

All restriction sites between EcoRI and HindIII are single-cut except AscI. Multiple gRNA expression cassettes cloned within the MCSs between two AscI sites can be subcloned into the AscI site of pFGC-pcoCas9 binary plasmid (Addgene plasmid#52256) through one-step cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC119-MCS was a gift from Jen Sheen (Addgene plasmid # 58807 ; http://n2t.net/addgene:58807 ; RRID:Addgene_58807)