pAM1
(Plasmid #51401)

• Purpose: Expresses truncated version of NLL-MetRS from endogenous MetG promoter
• Depositing Lab: David Tirrell
• Publication: Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):433-8. doi: 10.1073/pnas.1301740111. Epub 2013 Dec 17.

Backbone

• Vector backbone: pQE80 kan
• Modifications to backbone: Insert containing the MetG promoter was inserted into the Nhe1 cut site in the backbone of the pQE80 vector.

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MetRS
• Species: E coli
• Mutation: L13N, Y260L, H301L; Please note that a truncated version of MetRS is used for pAM1. The extra stop codon was deliberately put in the sequence and shortens the sequence from 677AA to 548AA.
• Entrez Gene: metG (a.k.a. b2114, ECK2107, JW2101)
• Promoter: MetG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (unknown if destroyed)
• 3′ cloning site: Nhe1 (unknown if destroyed)
• 5′ sequencing primer: 3primeUP
• 3′ sequencing primer: T0term-R

Resource Information

• Supplemental Documents:
  • insert sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry