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pAM1
(Plasmid #51401)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51401 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQE80 kan
  • Modifications to backbone
    Insert containing the MetG promoter was inserted into the Nhe1 cut site in the backbone of the pQE80 vector.

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MetRS
  • Species
    E coli
  • Mutation
    L13N, Y360L, H301L; Please note that a truncated version of MetRS is used for pAM1. The extra stop codon was deliberately put in the sequence and shortens the sequence from 677AA to 548AA.
  • Entrez Gene
    metG (a.k.a. b2114, ECK2107, JW2101)
  • Promoter MetG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (unknown if destroyed)
  • 3′ cloning site Nhe1 (unknown if destroyed)
  • 5′ sequencing primer 3primeUP
  • 3′ sequencing primer T0term-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM1 was a gift from David Tirrell (Addgene plasmid # 51401)
  • For your References section:

    Identification of secreted bacterial proteins by noncanonical amino acid tagging. Mahdavi A, Szychowski J, Ngo JT, Sweredoski MJ, Graham RL, Hess S, Schneewind O, Mazmanian SK, Tirrell DA. Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):433-8. doi: 10.1073/pnas.1301740111. Epub 2013 Dec 17. 10.1073/pnas.1301740111 PubMed 24347637