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(Plasmid #50832)


Item Catalog # Description Quantity Price (USD)
Plasmid 50832 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.


  • Vector backbone
  • Modifications to backbone
    derived from pFastBac-Dual from Invitrogen.
  • Vector type
    Insect Expression ; baculoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    two-copy Pyl tRNA cassette
  • Species
  • Promoter U6 and H1

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
  • Alt name
    pyrrolysyl-tRNA synthetase
  • Species
    Methanosarcina barkeri
  • Promoter CMV

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    two copy Pyl tRNA cassette
  • Species
  • Promoter U6 and H1

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcBac1.tR4-MbPyl was a gift from Peter Schultz (Addgene plasmid # 50832 ; ; RRID:Addgene_50832)
  • For your References section:

    Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Chatterjee A, Xiao H, Bollong M, Ai HW, Schultz PG. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. doi: 10.1073/pnas.1309584110. Epub 2013 Jul 1. 10.1073/pnas.1309584110 PubMed 23818609