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Life-Long Genetic and Functional Access to Neural Circuits Using Self-Inactivating Rabies Virus.
Ciabatti E, Gonzalez-Rueda A, Mariotti L, Morgese F, Tripodi M
Cell. 2017 Jul 1. pii: S0092-8674(17)30697-9. doi: 10.1016/j.cell.2017.06.014.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
99608pSAD-F3-NPEST-iCRE-2A-mCherryPESTSelf-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry
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99609pSAD-F3-NPEST-FLPo-2A-mCherryPESTSelf-inactivating DG-Rabies (SiR) expressing FLPo and destabilised mCherry
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99610pLenti-CMV-puro-2A-TEVpLentivirus expressing puromycin resistance gene and TEV protease
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102984pLenti-CMV-GFP-2A-oG-WPRELentivirus expressing eGFP and optimised Rabies Glycoprotein (oG)
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102985pAAV-CMV-FLEX-TVAmCherry-2A-oGAAV expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG) after CRE-mediated recombination
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102986pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPREAAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination
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102987pLenti-CMV-CAG-GFP-EnvALentivirus expressing eGFP and (EnvA-B19 Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies
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104330pAAV-CMV-TVAmCherry-2A-oGAAV constitutively expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG)
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104332pSAD-F3-NPEST-mCherrySelf-inactivating DG-Rabies (SiR) expressing mCherry
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