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pSAD-F3-NPEST-FLPo-2A-mCherryPEST
(Plasmid #99609)

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Ordering

This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 99609 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 99609-D50 50 μg of DNA in Tris buffer $495
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DNA 99609-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pSADdeltaG-F3
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Stbl2 or NEB Stable can also be used
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    FLPo

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Tag / Fusion Protein
    • PEST

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSAD-F3-NPEST-FLPo-2A-mCherryPEST was a gift from Marco Tripodi (Addgene plasmid # 99609 ; http://n2t.net/addgene:99609 ; RRID:Addgene_99609)

  • For your References section:

    Life-Long Genetic and Functional Access to Neural Circuits Using Self-Inactivating Rabies Virus. Ciabatti E, Gonzalez-Rueda A, Mariotti L, Morgese F, Tripodi M. Cell. 2017 Jul 1. pii: S0092-8674(17)30697-9. doi: 10.1016/j.cell.2017.06.014. 10.1016/j.cell.2017.06.014 PubMed 28689641
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