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PurposeMitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.
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Depositing Lab
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Depositing OrganizationShemyakin-Ovchinnikov Institute of Bioorganic Chemistry (INACTIVE)
Located in Russian Federation -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108120 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 108120-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 108120-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepC1
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Backbone manufacturerEvrogen
- Backbone size w/o insert (bp) 3937
- Total vector size (bp) 5578
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSypHer3s-IMS
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SpeciesSynthetic
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Insert Size (bp)1641
- Promoter CMV
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Tag
/ Fusion Protein
- ratiometricYFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ermakova Y. G., Pak V. V., Bogdanova Y. A., Kotlobay A. A., Yampolsky I.М., Shokhina A. G., A. S. Panova, R. Marygin, D. B. Staroverov, D. S. Bilan, H. Sies and V. V. Belousov “SypHer3s: genetically encoded fluorescent ratiometric probe with enhanced brightness and improved dynamic range”, Chemical Communications, 2017, upcoming publication
DNA (Catalog # 108120-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 108120-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC1-SypHer3s-IMS was a gift from Vsevolod Belousov (Addgene plasmid # 108120 ; http://n2t.net/addgene:108120 ; RRID:Addgene_108120) -
For your References section:
SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range. Ermakova YG, Pak VV, Bogdanova YA, Kotlobay AA, Yampolsky IV, Shokhina AG, Panova AS, Marygin RA, Staroverov DB, Bilan DS, Sies H, Belousov VV. Chem Commun (Camb). 2018 Mar 2. doi: 10.1039/c7cc08740c. 10.1039/c7cc08740c PubMed 29497715
