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pC1-SypHer3s-dmito
(Plasmid #108119)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108119 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 108119-D50 50 μg of DNA in Tris buffer $495
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DNA 108119-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pC1
  • Backbone manufacturer
    Evrogen
  • Backbone size w/o insert (bp) 3931
  • Total vector size (bp) 5539
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SypHer3s-dmito
  • Alt name
    mitochondrial SypHer3s; MLS1-2 SypHer3s
  • Species
    Synthetic
  • Insert Size (bp)
    1608
  • Promoter CMV
  • Tag / Fusion Protein
    • ratiometric YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Ermakova Y. G., Pak V. V., Bogdanova Y. A., Kotlobay A. A., Yampolsky I. М., Shokhina A. G., A. S. Panova, R. Marygin, D. B. Staroverov, D. S. Bilan, H. Sies and V. V. Belousov “SypHer3s: genetically encoded fluorescent ratiometric probe with enhanced brightness and improved dynamic range”, Chemical Communications, 2017, accepted publication

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC1-SypHer3s-dmito was a gift from Vsevolod Belousov (Addgene plasmid # 108119 ; http://n2t.net/addgene:108119 ; RRID:Addgene_108119)

  • For your References section:

    SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range. Ermakova YG, Pak VV, Bogdanova YA, Kotlobay AA, Yampolsky IV, Shokhina AG, Panova AS, Marygin RA, Staroverov DB, Bilan DS, Sies H, Belousov VV. Chem Commun (Camb). 2018 Mar 2. doi: 10.1039/c7cc08740c. 10.1039/c7cc08740c PubMed 29497715
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