SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range.
Ermakova YG, Pak VV, Bogdanova YA, Kotlobay AA, Yampolsky IV, Shokhina AG, Panova AS, Marygin RA, Staroverov DB, Bilan DS, Sies H, Belousov VV
Chem Commun (Camb). 2018 Mar 2. doi: 10.1039/c7cc08740c. PubMed Article
Ermakova YG, Pak VV, Bogdanova YA, Kotlobay AA, Yampolsky IV, Shokhina AG, Panova AS, Marygin RA, Staroverov DB, Bilan DS, Sies H, Belousov VV
Chem Commun (Camb). 2018 Mar 2. doi: 10.1039/c7cc08740c. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108118 | pC1-SypHer3s | Ratiometric pH probe with enhanced brightness for mammalian cells expression. |
| 108119 | pC1-SypHer3s-dmito | Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix. |
| 108120 | pC1-SypHer3s-IMS | Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression. |