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PurposeLight-controlled activation of expression
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Depositing Lab
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Depositing OrganizationUniversity of Helsinki, Institute of Biomedicine
Located in Finland -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 102583-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 102583-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSUBCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBphP1-VP16-T2A-NLS-GAL4-QPAS1
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's provided genbank file. The depositor noted that these discrepancies do NOT affect plasmid function.
New paper providing detailed protocol for using this plasmid- https://www.nature.com/articles/nprot.2018.022
DNA (Catalog # 102583-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 102583-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQP-T2A was a gift from Vladislav Verkhusha (Addgene plasmid # 102583 ; http://n2t.net/addgene:102583 ; RRID:Addgene_102583) -
For your References section:
Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Redchuk TA, Omelina ES, Chernov KG, Verkhusha VV. Nat Chem Biol. 2017 Mar 27. doi: 10.1038/nchembio.2343. 10.1038/nchembio.2343 PubMed 28346403
Map uploaded by the depositor.
