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PurposeRegulation of protein localization (near-infrared-blue-light-inducible shuttle)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102584 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepIRES2-EGFP
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's provided genbank file. The depositor noted that these discrepancies do NOT affect plasmid function.
New paper providing detailed protocol for using this plasmid- https://www.nature.com/articles/nprot.2018.022
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQP-iRIS was a gift from Vladislav Verkhusha (Addgene plasmid # 102584 ; http://n2t.net/addgene:102584 ; RRID:Addgene_102584) -
For your References section:
Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Redchuk TA, Omelina ES, Chernov KG, Verkhusha VV. Nat Chem Biol. 2017 Mar 27. doi: 10.1038/nchembio.2343. 10.1038/nchembio.2343 PubMed 28346403
Map uploaded by the depositor.
