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Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair.
Kato-Inui T, Takahashi G, Hsu S, Miyaoka Y
Nucleic Acids Res. 2018 May 18;46(9):4677-4688. doi: 10.1093/nar/gky264.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
108292pX459V2.0-eSpCas9(1.1)pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations
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108293pX459V2.0-SpCas9-HF1pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations
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108294pX459V2.0-HypaCas9pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations
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108295pX459V2.0-SpCas9-HF4pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations
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108296pX459V2.0+K848ApX459 V2.0 (Plasmid #62988) with the K848A mutation
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108297pX459V2.0-eSpCas9(1.1)+K810ApX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations
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108298pX459V2.0-eSpCas9(1.1)+K855ApX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations
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108299pX459V2.0-eSpCas9(1.1)+K810A+K855ApX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutations
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108300pX459V2.0+N497A+Q695A+Q926ApX459 V2.0 (Plasmid #62988) with the N497A, Q695A, and Q926A mutations
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108301pX330-SpCas9-HF1pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations
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108302pX330-HypaCas9pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations
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Recombinant Antibodies from Article