|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||108295||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDr. Feng Zhang's Lab
- Backbone size (bp) 9175
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pX459V2.0-SpCas9-HF4 was a gift from Yuichiro Miyaoka (Addgene plasmid # 108295 ; http://n2t.net/addgene:108295 ; RRID:Addgene_108295)
For your References section:Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair. Tomoko Kato-Inui, Gou Takahashi, Szuyin Hsu, Yuichiro Miyaoka. Nucleic Acids Research 10.1093/nar/gky264