Purpose(Empty Backbone) SpCas9-NG-mediated genome editing.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||171370||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 9175
Modifications to backbonepX459-SpCas9-NG was constructed by replacing the Cas9 coding sequence of pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid #62988) with that of pX330-SpCas9-NG (Addgene plasmid #117919) using AgeI (R0552, NEB, MA, USA) and FseI (R0588, NEB) restriction enzymes.
Vector typeMammalian Expression, CRISPR
- Promoter U6 for sgRNA; CBh for Cas9-2A-puroR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pX459-SpCas9-NG was a gift from Masahito Ikawa (Addgene plasmid # 171370 ; http://n2t.net/addgene:171370 ; RRID:Addgene_171370)
For your References section:Precise CAG repeat contraction in a Huntington's Disease mouse model is enabled by gene editing with SpCas9-NG. Oura S, Noda T, Morimura N, Hitoshi S, Nishimasu H, Nagai Y, Nureki O, Ikawa M. Commun Biol. 2021 Jun 23;4(1):771. doi: 10.1038/s42003-021-02304-w. 10.1038/s42003-021-02304-w PubMed 34163001