pX459-SpCas9-NG
(Plasmid
#171370)
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Purpose(Empty Backbone) SpCas9-NG-mediated genome editing.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171370 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepX459
- Backbone size (bp) 9175
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Modifications to backbonepX459-SpCas9-NG was constructed by replacing the Cas9 coding sequence of pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid #62988) with that of pX330-SpCas9-NG (Addgene plasmid #117919) using AgeI (R0552, NEB, MA, USA) and FseI (R0588, NEB) restriction enzymes.
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Vector typeMammalian Expression, CRISPR
- Promoter U6 for sgRNA; CBh for Cas9-2A-puroR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pX459-SpCas9-NG was a gift from Masahito Ikawa (Addgene plasmid # 171370 ; http://n2t.net/addgene:171370 ; RRID:Addgene_171370) -
For your References section:
Precise CAG repeat contraction in a Huntington's Disease mouse model is enabled by gene editing with SpCas9-NG. Oura S, Noda T, Morimura N, Hitoshi S, Nishimasu H, Nagai Y, Nureki O, Ikawa M. Commun Biol. 2021 Jun 23;4(1):771. doi: 10.1038/s42003-021-02304-w. 10.1038/s42003-021-02304-w PubMed 34163001
