164,674 results

1. pErCas12a EZ Clone

   Plasmid

   #132641

   Purpose

   Expresses ErCas12a and an sgRNA to target a region of interest

   Depositor

   Stephen Ekker

   Article

   Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

   Insert

   ErCas12a
   

   Use

   AAV and CRISPR

   Tags

   3x HA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Q926R (Please see depositor comments) and BsaI si…

   Promoter

   CMV Promoter

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. GPR142-Tango

   Plasmid

   #66319

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR142 (GPR142 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCEP4-001428gp160

   Plasmid

   #123255

   Purpose

   Mammalian expression plasmid for Env from the 001428 HIV-1 isolate

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (001428) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. HR_G3BP1-V5-APEX2-GFP

   Plasmid

   #105284

   Purpose

   homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

   Depositor

   Eugene Yeo

   Article

   Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

   Insert

   G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)
   

   Use

   Human homologous recombination donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_S100A13_WT_V5

   Plasmid

   #82975

   Purpose

   Gateway Donor vector containing S100A13, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, David Root, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   S100A13 (S100A13 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. IL1RN gRNA1

   Plasmid

   #113130

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for gRNA expression, RSV for GFP expression

   Available since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pExp-GB1

   Plasmid

   #112565

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-GB1 and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSPPac-hp

   Plasmid

   #48123

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

   Insert

   signal peptide of Pac
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV1)

   Viral Prep

   #26975-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   CaMKII

   Tags

   mCherry

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pCEP4-H031gp160

    Plasmid

    #123272

    Purpose

    Mammalian expression plasmid for Env from the H031 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (H031) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. IL1RN gRNA3

    Plasmid

    #113132

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI152-pLenti-SiT-Cas12a

    Plasmid

    #128405

    Purpose

    Express both AsCas12a and CRISPR arrays on single transcripts

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-Triplex
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xERRE/ERE-luciferase

    Plasmid

    #37852

    Depositor

    Rebecca Riggins

    Article

    Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

    Insert

    3 copies of ERRE/ERE upstream of luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXG237

    Plasmid

    #141281

    Purpose

    ATF4 translational reporter

    Depositor

    Martin Kampmann

    Article

    Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

    Insert

    uORF1/2 of ATF4 followed by mApple
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV HaloTag-LgBiT Vector

    Plasmid

    #236917

    Purpose

    Mammalian expression vector encoding HaloTag(R)-LgBiT for use in HiBiT cell lines

    Depositor

    Promega Corporation

    Has Service

    DNA

    Type

    Empty backbone

    Use

    Tags

    HaloTag (R)-LgBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pNL [NlucP/CEBP-RE/Hygro] Vector

    Plasmid

    #236832

    Purpose

    Express CEBP response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CEBP (CEBPA Human)
    

    Use

    Luciferase

    Tags

    NanoLuc (R)

    Expression

    Mutation

    None

    Promoter

    minP

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGL4 [luc2P/ATF4-RE/Hygro] Vector

    Plasmid

    #236816

    Purpose

    Express ATF4 response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    minP

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pIF1079

    Plasmid

    #237445

    Purpose

    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

    Depositor

    IIya Finkelstein

    Article

    Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/

    Inserts

    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, H1, and U6

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR TRE_firefly Luciferase_2A_mCherry-PGK_iRFP720

    Plasmid

    #223543

    Purpose

    Inducible firefly luciferase and mCherry; constitutive far-red iRFP720

    Depositor

    Jonathan Brunger

    Article

    Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

    Inserts

    Enhanced firefly luciferase
    mCherry
    iRFP720
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. IK349

    Plasmid

    #233655

    Purpose

    Dox-inducible red light-inducible Flp-C; Flp-397C-PhyA

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    Flp-397C-PhyA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    TRE3G

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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