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pFastBac1 Flag BRG1
(Plasmid #1957)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1957 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4775
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRG1
  • Alt name
    BAF190a
  • Species
    H. sapiens (human)
  • Entrez Gene
    SMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer Polyhedrin forward
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

A C-terminal Flag tag was added to BRG1 by PCR using a 3? primer designed to incorporate the FLAG sequence (DYKDDDDK) before the stop codon, followed by SalI and SpeI restriction sites. The PCR product was digested with BglII and SpeI and cloned into pBS/Brg1. The full-length Brg1 cDNA was then excised out of pBS/FL-Brg1 as a SalI fragment and cloned into pFastBac1 to generate pFastBac1/Brg1(Flag). (kingston #1306)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac1 Flag BRG1 was a gift from Robert Kingston (Addgene plasmid # 1957 ; http://n2t.net/addgene:1957 ; RRID:Addgene_1957)
  • For your References section:

    Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits. Phelan ML, Sif S, Narlikar GJ, Kingston RE. Mol Cell 1999 Feb;3(2):247-53. 10.1016/S1097-2765(00)80315-9 PubMed 10078207