pFastBac1 Flag BAF155 b
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1954||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4775
Vector typeInsect Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)4200
Entrez GeneSMARCC1 (a.k.a. BAF155, CRACC1, Rsc8, SRG3, SWI3)
/ Fusion Protein
- flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI/XhoI (destroyed during cloning)
- 3′ cloning site SphI (not destroyed)
- 5′ sequencing primer Polyhedrin forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
We constructed a composite full-length cDNA for hSWI3 (BAF155) using a 2.4 kb partial human cDNA that was isolated as described previously and a 1.8 kb RT-PCR-amplified fragment obtained from human liver poly(A) RNA. We consistently isolated the partial hSWI3 cDNA (2.4 kb) from several libraries. It appears that an adenine-rich stretch at the 3' end of these cDNAs hybridizes with the oligo(dt) primers used to create the first strand of the cDNA. An oligo that anneals in the sense direction to the partial cDNA, and an oligo that anneals to the 3' UTR of the highly related hSWI3 clone described previously, BAF155, were used to generate the missing coding sequences. The amplified fragment was inserted into the BamHI and SpeI sites of pBS-5'hSWI3, which contains the partial hSWI3 cDNA. The protein encoded by this complete ORF is highly related to BAF155, except for a few positions scattered throughout the protein. Most of these differences are substitutions of BAF155 sequences with the corresponding sequences of BAF170. To generate pFastBac1-BAF155, an XhoI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFastBac1 Flag BAF155 b was a gift from Robert Kingston (Addgene plasmid # 1954 ; http://n2t.net/addgene:1954 ; RRID:Addgene_1954)
For your References section:Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits. Phelan ML, Sif S, Narlikar GJ, Kingston RE. Mol Cell 1999 Feb;3(2):247-53. 10.1016/S1097-2765(00)80315-9 PubMed 10078207
Map uploaded by the depositor.