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A nanobody-based strategy for rapid and scalable purification of human protein complexes.
Stevens TA, Tomaleri GP, Hazu M, Wei S, Nguyen VN, DeKalb C, Voorhees RM, Pleiner T
Nat Protoc. 2024 Jan;19(1):127-158. doi: 10.1038/s41596-023-00904-w. Epub 2023 Nov 16. PubMed Article

Plasmids from Article

ID Plasmid Purpose
199346pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCSLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.
199347pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCSLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.
199348pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCSLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.
199349pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCSLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.
199350pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCSLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.
199351pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCSLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.
199352pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCSLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.
199353pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCSLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells.
199354pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCSLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells.
199355pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCSLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.
199356pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.
199357pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells.
199358pTS105 pHAGE2 CMVtetO2 MCS-TEV-22xGS-GFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-GFP-tagged protein in mammalian cells.
199359pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.
199360pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.
199361pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.
199362pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.
199363pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.
199364pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.
199365pTS112 pHAGE2 CMVtetO2 MCS-TEV-22xGS-Alfa-P2A-TagBFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-ALFA-P2A-BFP-tagged protein in mammalian cells.
199366pTS113 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-Alfa-P2A-TagBFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.
199367pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.
199368pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.
199369pTS116 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-Alfa-P2A-TagBFPLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.
199370pTS117 His14-Avi-45xGS-anti GFP nanobodyBacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)
199371pTS118 His14-Avi-28xGS-anti ALFA tag nanobodyBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)
199390pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobodyBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag
199391pTP341 pHAGE2 CMV TagBFP-3xFLAGLentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells
199392pTP924 pHAGE2 CMV GFPLentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

Antibodies from Article