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The oncogenic function of PLAGL2 is mediated via ASCL2 and IGF2 and a Wnt-independent mechanism in colorectal cancer.
Fischer AD, Veronese-Paniagua DA, Swaminathan S, Kashima H, Rubin DC, Madison BB
Am J Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 10.1152/ajpgi.00058.2022. PubMed Article

Plasmids from Article

ID Plasmid Purpose
133355BII-gR-PnGWPiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLS
133357BII-gR-PtW-eSpCas9Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
133358BII-gR-BtW-eSpCas9Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
133359BII-Sh-PnGWPiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS
133360BII-Sh-PnGW-LucPiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS
133361BII-Sh-PIR702WPiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS
133362BII-Sh-PIR702W-LucPiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS
133363BII-Sh-FUCCIPiggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter
133364BII-Sh-FUCCI-LucPiggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter
133366BII-Sh-PnGW-CMVPiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS
133367BII-Sh-PnGW-CMV-LucPiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS
133368BII-ChBtWPiggybac vector for constitutive expression
133369BII-ChBtW-PLAGL2Piggybac vector for constitutive expression
133370BII-ChBtW-eSpCas9Piggybac vector for constitutive expression
133371BII-ChBtW-iresFUCCIPiggybac vector for constitutive expression
133372BII-CBtWPiggybac vector for constitutive expression
133374BII-ChBtW-IGF2Piggybac vector for constitutive expression
133375BII-ChBtW-ASCL2Piggybac vector for constitutive expression
133376BII-ChBtW-CTNNB1_S33YPiggybac vector for constitutive expression
133377BII-ChPtinGWPiggybac vector for constitutive expression
133378BII-ChBtW-dnTCF4-ires-GFPPiggybac vector for constitutive expression
133379BII-ChPtWPiggybac vector for constitutive expression
133380BII-ChPtW-PLAGL2Piggybac vector for constitutive expression
133381BII-ChPtW-eSpCas9Piggybac vector for constitutive expression
133382BII-ChiNWPiggybac vector for constitutive expression
133383BII-ChiNW-IGF2Piggybac vector for constitutive expression
133384BII-ChiNW-ASCL2Piggybac vector for constitutive expression
133402BII-CMV-AfmW3APiggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

Antibodies from Article