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Taek Soon Lee Lab Plasmids


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Title Authors Publication
Identification and microbial production of a terpene-based advanced biofuel. Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold CJ, Lee TS Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Satoh Y, Tajima K, Munekata M, Keasling JD, Lee TS Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29.
Isopentenyl diphosphate (IPP)-bypass mevalonate pathways for isopentenol production. Kang A, George KW, Wang G, Baidoo E, Keasling JD, Lee TS Metab Eng. 2016 Mar;34:25-35. doi: 10.1016/j.ymben.2015.12.002. Epub 2015 Dec 17.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
35150pBbA5c-MevT-MBISAtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA (Saccharomyces cerevisiae)Bacterial Expression, Synthetic BiologyIdentification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pBbA5c Add to Cart
35151pBbA5c-MevT(CO)-MBIS(CO, ispA)AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA (Saccharomyces cerevisiae)Bacterial Expression, Synthetic BiologyIdentification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pBbA5c Add to Cart
35152pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA (Saccharomyces cerevisiae)Bacterial Expression, Synthetic BiologyIdentification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pBbA5c Add to Cart
35153pTrcAgBIS (CO)Bisabolene SynthaseBacterial Expression, Synthetic BiologyIdentification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pTrc99a Add to Cart
35524pRSLeu2d-BISoptBisabolene SynthaseYeast Expression, Synthetic BiologyIdentification and microbial production of a terpene-based advanced biofuel. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. pRS425ADS-Leu2d Add to Cart
47048pJBEI-6409Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology ; BglBrickMetabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli pBbA5c Add to Cart
47049pJBEI-6410Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene (Saccharomyces cerevisiae)Bacterial Expression, Synthetic Biology ; BglBrickMetabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli pBbA5a Add to Cart
47050pJBEI-6411ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase (Other)Bacterial Expression, Synthetic Biology ; BglBrickMetabolic engineering of Escherichia coli for limonene and perillyl alcohol production. Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004. BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli pBbB8k Add to Cart
59945pBbE1k-2dihydropterine reductase, pterin-4a-carbinolamine dehydrataseEngineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29. dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human pBbE1k Add to Cart
71722pBbE1k-3dihydropterine reductase (Homo sapiens), pterin-4a-carbinolamine dehydratase (Homo sapiens)Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29. tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human pBbE1k QDPR DHPR, PKU2, SDR33C1 Add to Cart
71723pBbE1k-DDCdopa decarboxylase (Other)Engineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol. Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. Epub 2012 Aug 29. dopa decarboxylase from pig pBbE1k Add to Cart
81013ARK2aaMevTo-BBa1002-pTrc-Mkco-aphA, and PMDIsopentenyl diphosphate (IPP)-bypass mevalonate pathways for isopentenol production. Metab Eng. 2016 Mar;34:25-35. doi: 10.1016/j.ymben.2015.12.002. Epub 2015 Dec 17. E.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366) p15A and ColE1 Add to Cart
81016ARK1aMevTo-BBa1002-pTrc-Mkco-PMKco, PMD-NudBIsopentenyl diphosphate (IPP)-bypass mevalonate pathways for isopentenol production. Metab Eng. 2016 Mar;34:25-35. doi: 10.1016/j.ymben.2015.12.002. Epub 2015 Dec 17. E.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365) p15A and ColE1 Add to Cart
81017ARK2aMevTo-BBa1002-pTrc-Mkco, PMDIsopentenyl diphosphate (IPP)-bypass mevalonate pathways for isopentenol production. Metab Eng. 2016 Mar;34:25-35. doi: 10.1016/j.ymben.2015.12.002. Epub 2015 Dec 17. E.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366) p15A and ColE1 Add to Cart